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General information, events and/or links.

 

 

Thermo Fisher Scientific

Group information, relevant EV research and instrumentation

Group name

Thermo Fisher Scientific, Life Sciences Solutions, Synthetic Biology, Norway

Affilitation

 

Ullernchausséen 52, 0379 Oslo, Norway

Link to homepage: www.thermofisher.com

 

 

Name

E-mail

Phone

Steering group member

Axl Neurauter

Axl.neurauter@thermofisher.com

+47 90 18 93 65

Deputy representative

Malin Karlsson

Malin.karlsson@thermofisher.com

+47 97 47 54 47

Group members

Bente Kierulf

Bente.kierulf@thermofisher.com

+47 93 40 13 55

Ketil Winther Pedersen

Ketil.pedersen@thermofisher.com

+47 91 18 14 78

Erlend Ragnhildstveit

Erlend.ragnhildstveit@thermofisher.com

+47 95 40 34 50

 

Scientific background/relevant EV research:

Life Technologies Inc is a biotech company delivering products and services into life science research, diagnostics and cell based therapy. The short term focus for our exosome/microvesicle initiative is development of reagents and methods for generic and specific isolation and analysis of exosomes/microvesicles with in life science research. The longer term goal is to explore the opportunities for exosome/microvesicle based diagnostics. 

 

In 2012/Q1 2013 Life Technologies has launched 7 products for exosome/microvesicle research:

-    products for generic isolation of exosomes/microvesicles from cell culture and human serum

-    products for specific isolation/detection of exosome/microvesicle sub-populations from cell  
culture (CD63 and Streptavidin magnetic beads)

-    one product for isolation of nucleic acid (and protein) from exosomes/microvesicles (Trizol)

-    two products for isolation of proteins from exosomes/microvesicles (Prot A/G magnetic beads)

 

Ongoing research includes standardization and expand the product portfolio for specific isolation/detection of exosome/microvesicle sub-populations targeting molecules such as CD9 and CD81. Included in standardization work is:

-    optimized production of exosome enriched cell culture supernatants

-    methods for flow analysis of exosomes

In additions, analysis methods such as EM and western have been established better characterize the exosomes/microvesicles and their content, while methods such as MS, qRT-PCR and sequencing are being explored.

 

Future work will include transition to body fluids such as serum/plasma and urine as sources for exosome/microvesicle isolation/detection.  

 

Instrumentation (relevant for EV research):

  • NanoSight (LM10)
  • Western blotting (gel, iBlot = dry blotting system)
  • ChemiDoc MP Imager (fluorescence and bright light gel/blot Imager)
  • BD LSR II flow instrument
  • Floid (fluorescence microscope with very easy user interface and image capture capabilities)
  • Fluorescence microscope (Nikon)
  • Qbit and NanoDrop (very low volume protein and NA measurements)
  • EM expertise (do not have instrument but buy access at UiO - IMBV, Prof. Norbert Roos)
  • Beckman J2-21 Centrifuge (fix angle); JA-20 rotor 48400xg 400 ml, JA-10 rotor 17700xg 3000 ml
  • Applied Biosystems 7500 real time PCR system
  • Wallac Victor2 1420 Multicounter (delayed fluorescence platform)
  • Biotek Synergy 4 (spectrophotometer)

 

 

Date 

August 21st, 2015