General information, events and/or links.



The Blood Cell Research Group

Group information, relevant EV research and instrumentation

Group name

The Blood Cell Research Group, BCG



Dept. of Medical Biochemistry, OUH, Ullevål

Link to homepage:






Leader of RRNEV

Reidun Øvstebø

22119491/ 48154308

Deputy representative

Kari Bente Foss Haug

22117759/ 93047410 

RRNEV administrator

Beate Vestad

22119490/ 92431223

Group members

Peter Kierulf


Jens Petter Berg

23027251/ 48496950

Marit Hellum

22119483/ 93411652

Berit Brusletto 

23016424/ 92032335

Hans Christian D. Aass

22119483/ 98699302

Carola Henriksson

22118356/ 23074134

Anne Marie S. Trøseid 

22119490/ 90858386

Trude Aspelin


Tine Hiorth Schøyen


Lilly Alice Skaaraas



Scientific background/relevant EV research:

The Blood Cell Research Group studies mechanisms regulating inflammation and coagulation. The research aims to disclose the roles of human monocytes and monocyte-derived microparticles in the initiation of coagulation and for cytokines in inflammation using purified human monocytes and whole blood models. The Blood Cell Research Group is the coordinating group in the Regional Research Network on Extracellular Vesicles (RRNEV) ( The group has a specific focus on methods for isolating and characterization of extracellular vesicles in blood.


Other EV-related research projects:

“On the properties of monocytes and monocyte-derived MPs”, phD project Hans Christian D. Aass, supervised by Carola Henriksson, Peter Kierulf and Jens P. Berg. Aims of this study are to examine the steps required to optimally study TF-positive MPs derived from monocytes in culture by flow cytometry, and to study effects of membrane-bound LPS on monocytes and monocyte-derived MPs on the expression and activity of TF.

“Monocytes and microparticles in experimental and clinical settings of meningococcal sepsis – with special focus on tissue factor (TF), phD project Marit Hellum, supervised by Carola Henriksson, Reidun Øvstebø and Jens P. Berg. Aims of this study are to evaluate preanalytical procedures and assay technologies for measuring MP-associated TF activity, to describe TF-dependent procoagulant properties and content (RNA and/or miRNA) of MPs in plasmas from patients with meningitis or sepsis, and to study the TF-inducing capacity of lipid A mutated Neisseria meningitidis.

“Development of diagnostic and prognostic molecular markers for ovarian cancer”
, follow up from the PhD project by Bente Vilming Elgaaen. Global mRNA and miRNA expression profiles in tumor cells from ovarian carcinoma patients, classified into different clinical subgroups, have been compared to healthy controls. To search for potential blood born biomarkers, differentially expressed RNAs will be compared to circulating miRNA levels in the EV fraction of plasma samples from the study group. Kari Bente Foss Haug, PhD, is working to design optimized procedures for isolation of circulating EVs.

- Collaboration project with Michal Mielnik at SINTEF MiNaLab, to develop a device for isolating EVs from biological fluids.


“Understanding salivary gland function” .

  • Proteomic studies in whole saliva and EVs isolated from whole saliva, from healthy persons and patients with Sjøgrens syndrome. Collaboration with Professor Janicke Cecilie Liaaen Jensen, Faculty of Dentistry, University of Oslo.
  • Exosomes in oral tissue differentiation and dedifferentiation: their presence, uptake and effect during organ development and cancer development. Collaboration with Professor Hilde Galtung and Tine Søland, Faculty of Dentistry, University of Oslo.


External collaborators:

  • Janicke Cecilie Liaaen Jensen, Faculty of Dentistry, University of Oslo, Norway.
  • Bård Smedsrød, Institute of Medical Biology, University of Tromsø, Norway
  • Rienk Nieuwland, Department of Clinical Chemistry, Academic Medical Center, Amsterdam, the Netherlands.
  • Michal Mielnik, SINTEF MiNaLab, Oslo, Norway.



Instrumentation (relevant for EV research):

  • Flow cytometry core facility
    • Flow cytometers (FACS Diva, FACS ARIA, and Accuri C6)
    • Luminex – multiplex analysis of intra-and-extracellular proteins (+ RNA/DNA)
  • Affymetrix  microarray core facility – gene expression studies (RNA, miRNA)
  • Nikon elipse Ti, inverted fluorescence microscope
  • Nanosight NS500 – sizing and enumeration of EVs
  • Qiacube – automated isolation of DNA, RNA and protein with Qiagen columns
  • MagnaPure – automated isolation of DNA, RNA and protein
  • ViiA 7 –  RT-qPCR on single genes or LDA cards (RNA, miRNA)
  • LightCycler – RT-qPCR
  • Several small blockcyclers  for PCR
  • Nanodrop ND-1000 and ND-3300 (fluorescence) – quantification of nucleic acids (and proteins)
  • Bioanalyzer – quality control of nucleic acids
  • Qubit – quantification of proteins and nucleic acids
  • Coagulation assays for thrombin – and fibrin generation, including CAT (Calibrated Automated Thrombogram)
  • Bolt® iBlot® 2 Western Blot System
  • Beckman Coulter Elutriator (Avanti J-26 XP)- isolation/ purification of large amount of cells




August 24th, 2015